gr 1 Search Results


ly6gpe  (Miltenyi Biotec)
95
Miltenyi Biotec ly6gpe
Ly6gpe, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/gr+1/pm37506784-83-19-27?v=Miltenyi+Biotec
Average 95 stars, based on 1 article reviews
ly6gpe - by Bioz Stars, 2026-07
95/100 stars
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gr1  (R&D Systems)
94
R&D Systems gr1
Mice were exposed to 7.5 Gy for 1 week to induce intestinal inflammation. A Photomicrographs of colon sections, histological grades, colon lengths, body weights, and survival rates for mice 21 days after irradiation. Scale bar = 100 μm B Representative dot plots of the gating strategy used to identify CD11b + <t>/GR1</t> + cells from the spleens and intestines of mice 21 days after irradiation. C Representative immunofluorescence images of spleen and intestine stained for CD11b (green), GR1 (red), and CD11b + /GR1 + (yellow) cells. DAPI staining (blue) was used to determine the number of nuclei. Scale bar = 100 μm D CFSE-labeled CD3 + T cells were co‑cultured with splenic MDSCs and stimulated using anti-CD3 and anti-CD28 monoclonal antibodies. The cells were harvested and analyzed after 7 days. CFSE dilution demonstrated that T-cell proliferation was inhibited by MDSCs (left) and that this inhibition occurred in a dose-dependent manner (right). E The two MDSC subtypes, namely gMDSCs (CD11b + , Ly6G + , and Ly6C low ) and mMDSCs (CD11b + , Ly6G - , and Ly6C high ), were identified and analyzed by flow cytometry from spleen and intestine samples. The data are represented as the mean ± SD (n = 8). * P < 0.05, ** P < 0.01, *** P < 0.001 vs. control. IR irradiation.
Gr1, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/gr+1/pmc08417278-260-36-37?v=R%26D+Systems
Average 94 stars, based on 1 article reviews
gr1 - by Bioz Stars, 2026-07
94/100 stars
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gr 1  (Cedarlane)
85
Cedarlane gr 1
Mice were exposed to 7.5 Gy for 1 week to induce intestinal inflammation. A Photomicrographs of colon sections, histological grades, colon lengths, body weights, and survival rates for mice 21 days after irradiation. Scale bar = 100 μm B Representative dot plots of the gating strategy used to identify CD11b + <t>/GR1</t> + cells from the spleens and intestines of mice 21 days after irradiation. C Representative immunofluorescence images of spleen and intestine stained for CD11b (green), GR1 (red), and CD11b + /GR1 + (yellow) cells. DAPI staining (blue) was used to determine the number of nuclei. Scale bar = 100 μm D CFSE-labeled CD3 + T cells were co‑cultured with splenic MDSCs and stimulated using anti-CD3 and anti-CD28 monoclonal antibodies. The cells were harvested and analyzed after 7 days. CFSE dilution demonstrated that T-cell proliferation was inhibited by MDSCs (left) and that this inhibition occurred in a dose-dependent manner (right). E The two MDSC subtypes, namely gMDSCs (CD11b + , Ly6G + , and Ly6C low ) and mMDSCs (CD11b + , Ly6G - , and Ly6C high ), were identified and analyzed by flow cytometry from spleen and intestine samples. The data are represented as the mean ± SD (n = 8). * P < 0.05, ** P < 0.01, *** P < 0.001 vs. control. IR irradiation.
Gr 1, supplied by Cedarlane, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/gr+1/pmc02193574-68-16-21?v=Cedarlane
Average 85 stars, based on 1 article reviews
gr 1 - by Bioz Stars, 2026-07
85/100 stars
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anti mouse gr 1  (Bio X Cell)
96
Bio X Cell anti mouse gr 1
Mice were exposed to 7.5 Gy for 1 week to induce intestinal inflammation. A Photomicrographs of colon sections, histological grades, colon lengths, body weights, and survival rates for mice 21 days after irradiation. Scale bar = 100 μm B Representative dot plots of the gating strategy used to identify CD11b + <t>/GR1</t> + cells from the spleens and intestines of mice 21 days after irradiation. C Representative immunofluorescence images of spleen and intestine stained for CD11b (green), GR1 (red), and CD11b + /GR1 + (yellow) cells. DAPI staining (blue) was used to determine the number of nuclei. Scale bar = 100 μm D CFSE-labeled CD3 + T cells were co‑cultured with splenic MDSCs and stimulated using anti-CD3 and anti-CD28 monoclonal antibodies. The cells were harvested and analyzed after 7 days. CFSE dilution demonstrated that T-cell proliferation was inhibited by MDSCs (left) and that this inhibition occurred in a dose-dependent manner (right). E The two MDSC subtypes, namely gMDSCs (CD11b + , Ly6G + , and Ly6C low ) and mMDSCs (CD11b + , Ly6G - , and Ly6C high ), were identified and analyzed by flow cytometry from spleen and intestine samples. The data are represented as the mean ± SD (n = 8). * P < 0.05, ** P < 0.01, *** P < 0.001 vs. control. IR irradiation.
Anti Mouse Gr 1, supplied by Bio X Cell, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/gr+1/pmc07811063-258-12-15?v=Bio+X+Cell
Average 96 stars, based on 1 article reviews
anti mouse gr 1 - by Bioz Stars, 2026-07
96/100 stars
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anti gr1 antibody  (Miltenyi Biotec)
93
Miltenyi Biotec anti gr1 antibody
Mice were exposed to 7.5 Gy for 1 week to induce intestinal inflammation. A Photomicrographs of colon sections, histological grades, colon lengths, body weights, and survival rates for mice 21 days after irradiation. Scale bar = 100 μm B Representative dot plots of the gating strategy used to identify CD11b + <t>/GR1</t> + cells from the spleens and intestines of mice 21 days after irradiation. C Representative immunofluorescence images of spleen and intestine stained for CD11b (green), GR1 (red), and CD11b + /GR1 + (yellow) cells. DAPI staining (blue) was used to determine the number of nuclei. Scale bar = 100 μm D CFSE-labeled CD3 + T cells were co‑cultured with splenic MDSCs and stimulated using anti-CD3 and anti-CD28 monoclonal antibodies. The cells were harvested and analyzed after 7 days. CFSE dilution demonstrated that T-cell proliferation was inhibited by MDSCs (left) and that this inhibition occurred in a dose-dependent manner (right). E The two MDSC subtypes, namely gMDSCs (CD11b + , Ly6G + , and Ly6C low ) and mMDSCs (CD11b + , Ly6G - , and Ly6C high ), were identified and analyzed by flow cytometry from spleen and intestine samples. The data are represented as the mean ± SD (n = 8). * P < 0.05, ** P < 0.01, *** P < 0.001 vs. control. IR irradiation.
Anti Gr1 Antibody, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/gr+1/pm39273035-109-7-9?v=Miltenyi+Biotec
Average 93 stars, based on 1 article reviews
anti gr1 antibody - by Bioz Stars, 2026-07
93/100 stars
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rat anti mouse gr 1 ly 6g  (R&D Systems)
94
R&D Systems rat anti mouse gr 1 ly 6g
Mice were exposed to 7.5 Gy for 1 week to induce intestinal inflammation. A Photomicrographs of colon sections, histological grades, colon lengths, body weights, and survival rates for mice 21 days after irradiation. Scale bar = 100 μm B Representative dot plots of the gating strategy used to identify CD11b + <t>/GR1</t> + cells from the spleens and intestines of mice 21 days after irradiation. C Representative immunofluorescence images of spleen and intestine stained for CD11b (green), GR1 (red), and CD11b + /GR1 + (yellow) cells. DAPI staining (blue) was used to determine the number of nuclei. Scale bar = 100 μm D CFSE-labeled CD3 + T cells were co‑cultured with splenic MDSCs and stimulated using anti-CD3 and anti-CD28 monoclonal antibodies. The cells were harvested and analyzed after 7 days. CFSE dilution demonstrated that T-cell proliferation was inhibited by MDSCs (left) and that this inhibition occurred in a dose-dependent manner (right). E The two MDSC subtypes, namely gMDSCs (CD11b + , Ly6G + , and Ly6C low ) and mMDSCs (CD11b + , Ly6G - , and Ly6C high ), were identified and analyzed by flow cytometry from spleen and intestine samples. The data are represented as the mean ± SD (n = 8). * P < 0.05, ** P < 0.01, *** P < 0.001 vs. control. IR irradiation.
Rat Anti Mouse Gr 1 Ly 6g, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/gr+1/pm20631308-53-53-57?v=R%26D+Systems
Average 94 stars, based on 1 article reviews
rat anti mouse gr 1 ly 6g - by Bioz Stars, 2026-07
94/100 stars
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anti mouse ly 6g clone 1a8 biotin  (Miltenyi Biotec)
95
Miltenyi Biotec anti mouse ly 6g clone 1a8 biotin
Mice were exposed to 7.5 Gy for 1 week to induce intestinal inflammation. A Photomicrographs of colon sections, histological grades, colon lengths, body weights, and survival rates for mice 21 days after irradiation. Scale bar = 100 μm B Representative dot plots of the gating strategy used to identify CD11b + <t>/GR1</t> + cells from the spleens and intestines of mice 21 days after irradiation. C Representative immunofluorescence images of spleen and intestine stained for CD11b (green), GR1 (red), and CD11b + /GR1 + (yellow) cells. DAPI staining (blue) was used to determine the number of nuclei. Scale bar = 100 μm D CFSE-labeled CD3 + T cells were co‑cultured with splenic MDSCs and stimulated using anti-CD3 and anti-CD28 monoclonal antibodies. The cells were harvested and analyzed after 7 days. CFSE dilution demonstrated that T-cell proliferation was inhibited by MDSCs (left) and that this inhibition occurred in a dose-dependent manner (right). E The two MDSC subtypes, namely gMDSCs (CD11b + , Ly6G + , and Ly6C low ) and mMDSCs (CD11b + , Ly6G - , and Ly6C high ), were identified and analyzed by flow cytometry from spleen and intestine samples. The data are represented as the mean ± SD (n = 8). * P < 0.05, ** P < 0.01, *** P < 0.001 vs. control. IR irradiation.
Anti Mouse Ly 6g Clone 1a8 Biotin, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/gr+1/pmc02937183-289-10-25?v=Miltenyi+Biotec
Average 95 stars, based on 1 article reviews
anti mouse ly 6g clone 1a8 biotin - by Bioz Stars, 2026-07
95/100 stars
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clone 1a8  (Bio X Cell)
95
Bio X Cell clone 1a8
Mice were exposed to 7.5 Gy for 1 week to induce intestinal inflammation. A Photomicrographs of colon sections, histological grades, colon lengths, body weights, and survival rates for mice 21 days after irradiation. Scale bar = 100 μm B Representative dot plots of the gating strategy used to identify CD11b + <t>/GR1</t> + cells from the spleens and intestines of mice 21 days after irradiation. C Representative immunofluorescence images of spleen and intestine stained for CD11b (green), GR1 (red), and CD11b + /GR1 + (yellow) cells. DAPI staining (blue) was used to determine the number of nuclei. Scale bar = 100 μm D CFSE-labeled CD3 + T cells were co‑cultured with splenic MDSCs and stimulated using anti-CD3 and anti-CD28 monoclonal antibodies. The cells were harvested and analyzed after 7 days. CFSE dilution demonstrated that T-cell proliferation was inhibited by MDSCs (left) and that this inhibition occurred in a dose-dependent manner (right). E The two MDSC subtypes, namely gMDSCs (CD11b + , Ly6G + , and Ly6C low ) and mMDSCs (CD11b + , Ly6G - , and Ly6C high ), were identified and analyzed by flow cytometry from spleen and intestine samples. The data are represented as the mean ± SD (n = 8). * P < 0.05, ** P < 0.01, *** P < 0.001 vs. control. IR irradiation.
Clone 1a8, supplied by Bio X Cell, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/gr+1/10__1158_slash_2767___9764__crc___25___0509-74-28-30?v=Bio+X+Cell
Average 95 stars, based on 1 article reviews
clone 1a8 - by Bioz Stars, 2026-07
95/100 stars
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antibodies against mouse  (Bio-Rad)
93
Bio-Rad antibodies against mouse
Mice were exposed to 7.5 Gy for 1 week to induce intestinal inflammation. A Photomicrographs of colon sections, histological grades, colon lengths, body weights, and survival rates for mice 21 days after irradiation. Scale bar = 100 μm B Representative dot plots of the gating strategy used to identify CD11b + <t>/GR1</t> + cells from the spleens and intestines of mice 21 days after irradiation. C Representative immunofluorescence images of spleen and intestine stained for CD11b (green), GR1 (red), and CD11b + /GR1 + (yellow) cells. DAPI staining (blue) was used to determine the number of nuclei. Scale bar = 100 μm D CFSE-labeled CD3 + T cells were co‑cultured with splenic MDSCs and stimulated using anti-CD3 and anti-CD28 monoclonal antibodies. The cells were harvested and analyzed after 7 days. CFSE dilution demonstrated that T-cell proliferation was inhibited by MDSCs (left) and that this inhibition occurred in a dose-dependent manner (right). E The two MDSC subtypes, namely gMDSCs (CD11b + , Ly6G + , and Ly6C low ) and mMDSCs (CD11b + , Ly6G - , and Ly6C high ), were identified and analyzed by flow cytometry from spleen and intestine samples. The data are represented as the mean ± SD (n = 8). * P < 0.05, ** P < 0.01, *** P < 0.001 vs. control. IR irradiation.
Antibodies Against Mouse, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/gr+1/bio_rxiv__2021__12__08__471876-178-4-29?v=Bio-Rad
Average 93 stars, based on 1 article reviews
antibodies against mouse - by Bioz Stars, 2026-07
93/100 stars
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monoclonal antibody  (Rockland Immunochemicals)
91
Rockland Immunochemicals monoclonal antibody
Mice were exposed to 7.5 Gy for 1 week to induce intestinal inflammation. A Photomicrographs of colon sections, histological grades, colon lengths, body weights, and survival rates for mice 21 days after irradiation. Scale bar = 100 μm B Representative dot plots of the gating strategy used to identify CD11b + <t>/GR1</t> + cells from the spleens and intestines of mice 21 days after irradiation. C Representative immunofluorescence images of spleen and intestine stained for CD11b (green), GR1 (red), and CD11b + /GR1 + (yellow) cells. DAPI staining (blue) was used to determine the number of nuclei. Scale bar = 100 μm D CFSE-labeled CD3 + T cells were co‑cultured with splenic MDSCs and stimulated using anti-CD3 and anti-CD28 monoclonal antibodies. The cells were harvested and analyzed after 7 days. CFSE dilution demonstrated that T-cell proliferation was inhibited by MDSCs (left) and that this inhibition occurred in a dose-dependent manner (right). E The two MDSC subtypes, namely gMDSCs (CD11b + , Ly6G + , and Ly6C low ) and mMDSCs (CD11b + , Ly6G - , and Ly6C high ), were identified and analyzed by flow cytometry from spleen and intestine samples. The data are represented as the mean ± SD (n = 8). * P < 0.05, ** P < 0.01, *** P < 0.001 vs. control. IR irradiation.
Monoclonal Antibody, supplied by Rockland Immunochemicals, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/gr+1/pmc10015250-58-47-49?v=Rockland+Immunochemicals
Average 91 stars, based on 1 article reviews
monoclonal antibody - by Bioz Stars, 2026-07
91/100 stars
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fitc anti mouse ly 6g ly 6c gr 1 antibody  (Elabscience Biotechnology)
94
Elabscience Biotechnology fitc anti mouse ly 6g ly 6c gr 1 antibody
Mice were exposed to 7.5 Gy for 1 week to induce intestinal inflammation. A Photomicrographs of colon sections, histological grades, colon lengths, body weights, and survival rates for mice 21 days after irradiation. Scale bar = 100 μm B Representative dot plots of the gating strategy used to identify CD11b + <t>/GR1</t> + cells from the spleens and intestines of mice 21 days after irradiation. C Representative immunofluorescence images of spleen and intestine stained for CD11b (green), GR1 (red), and CD11b + /GR1 + (yellow) cells. DAPI staining (blue) was used to determine the number of nuclei. Scale bar = 100 μm D CFSE-labeled CD3 + T cells were co‑cultured with splenic MDSCs and stimulated using anti-CD3 and anti-CD28 monoclonal antibodies. The cells were harvested and analyzed after 7 days. CFSE dilution demonstrated that T-cell proliferation was inhibited by MDSCs (left) and that this inhibition occurred in a dose-dependent manner (right). E The two MDSC subtypes, namely gMDSCs (CD11b + , Ly6G + , and Ly6C low ) and mMDSCs (CD11b + , Ly6G - , and Ly6C high ), were identified and analyzed by flow cytometry from spleen and intestine samples. The data are represented as the mean ± SD (n = 8). * P < 0.05, ** P < 0.01, *** P < 0.001 vs. control. IR irradiation.
Fitc Anti Mouse Ly 6g Ly 6c Gr 1 Antibody, supplied by Elabscience Biotechnology, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/gr+1/pm41776053-99-103-108?v=Elabscience+Biotechnology
Average 94 stars, based on 1 article reviews
fitc anti mouse ly 6g ly 6c gr 1 antibody - by Bioz Stars, 2026-07
94/100 stars
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blue 130 102 145  (Miltenyi Biotec)
94
Miltenyi Biotec blue 130 102 145
Mice were exposed to 7.5 Gy for 1 week to induce intestinal inflammation. A Photomicrographs of colon sections, histological grades, colon lengths, body weights, and survival rates for mice 21 days after irradiation. Scale bar = 100 μm B Representative dot plots of the gating strategy used to identify CD11b + <t>/GR1</t> + cells from the spleens and intestines of mice 21 days after irradiation. C Representative immunofluorescence images of spleen and intestine stained for CD11b (green), GR1 (red), and CD11b + /GR1 + (yellow) cells. DAPI staining (blue) was used to determine the number of nuclei. Scale bar = 100 μm D CFSE-labeled CD3 + T cells were co‑cultured with splenic MDSCs and stimulated using anti-CD3 and anti-CD28 monoclonal antibodies. The cells were harvested and analyzed after 7 days. CFSE dilution demonstrated that T-cell proliferation was inhibited by MDSCs (left) and that this inhibition occurred in a dose-dependent manner (right). E The two MDSC subtypes, namely gMDSCs (CD11b + , Ly6G + , and Ly6C low ) and mMDSCs (CD11b + , Ly6G - , and Ly6C high ), were identified and analyzed by flow cytometry from spleen and intestine samples. The data are represented as the mean ± SD (n = 8). * P < 0.05, ** P < 0.01, *** P < 0.001 vs. control. IR irradiation.
Blue 130 102 145, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/gr+1/us12564643-748-8-9?v=Miltenyi+Biotec
Average 94 stars, based on 1 article reviews
blue 130 102 145 - by Bioz Stars, 2026-07
94/100 stars
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Image Search Results


Mice were exposed to 7.5 Gy for 1 week to induce intestinal inflammation. A Photomicrographs of colon sections, histological grades, colon lengths, body weights, and survival rates for mice 21 days after irradiation. Scale bar = 100 μm B Representative dot plots of the gating strategy used to identify CD11b + /GR1 + cells from the spleens and intestines of mice 21 days after irradiation. C Representative immunofluorescence images of spleen and intestine stained for CD11b (green), GR1 (red), and CD11b + /GR1 + (yellow) cells. DAPI staining (blue) was used to determine the number of nuclei. Scale bar = 100 μm D CFSE-labeled CD3 + T cells were co‑cultured with splenic MDSCs and stimulated using anti-CD3 and anti-CD28 monoclonal antibodies. The cells were harvested and analyzed after 7 days. CFSE dilution demonstrated that T-cell proliferation was inhibited by MDSCs (left) and that this inhibition occurred in a dose-dependent manner (right). E The two MDSC subtypes, namely gMDSCs (CD11b + , Ly6G + , and Ly6C low ) and mMDSCs (CD11b + , Ly6G - , and Ly6C high ), were identified and analyzed by flow cytometry from spleen and intestine samples. The data are represented as the mean ± SD (n = 8). * P < 0.05, ** P < 0.01, *** P < 0.001 vs. control. IR irradiation.

Journal: Cell Death & Disease

Article Title: Expansion of monocytic myeloid-derived suppressor cells ameliorated intestinal inflammatory response by radiation through SOCS3 expression

doi: 10.1038/s41419-021-04103-x

Figure Lengend Snippet: Mice were exposed to 7.5 Gy for 1 week to induce intestinal inflammation. A Photomicrographs of colon sections, histological grades, colon lengths, body weights, and survival rates for mice 21 days after irradiation. Scale bar = 100 μm B Representative dot plots of the gating strategy used to identify CD11b + /GR1 + cells from the spleens and intestines of mice 21 days after irradiation. C Representative immunofluorescence images of spleen and intestine stained for CD11b (green), GR1 (red), and CD11b + /GR1 + (yellow) cells. DAPI staining (blue) was used to determine the number of nuclei. Scale bar = 100 μm D CFSE-labeled CD3 + T cells were co‑cultured with splenic MDSCs and stimulated using anti-CD3 and anti-CD28 monoclonal antibodies. The cells were harvested and analyzed after 7 days. CFSE dilution demonstrated that T-cell proliferation was inhibited by MDSCs (left) and that this inhibition occurred in a dose-dependent manner (right). E The two MDSC subtypes, namely gMDSCs (CD11b + , Ly6G + , and Ly6C low ) and mMDSCs (CD11b + , Ly6G - , and Ly6C high ), were identified and analyzed by flow cytometry from spleen and intestine samples. The data are represented as the mean ± SD (n = 8). * P < 0.05, ** P < 0.01, *** P < 0.001 vs. control. IR irradiation.

Article Snippet: The tissue sections were incubated with primary antibodies against CD11b (CST, 17800, 1:100 dilution, IL6 (CST, 12912, 1:100 dilution), IL10 (Abcam, ab192271, 1:100 dilution), and SOCS3 (Abcam, ab16030, 1:100 dilution), phospho-STAT3 (CST, 9145, 1:100 dilution), and GR1 (R&D Systems, MN, USA, MAB1037-100, 1:100 dilution) overnight at 4 °C.

Techniques: Irradiation, Immunofluorescence, Staining, Labeling, Bioprocessing, Inhibition, Flow Cytometry, Control

Mice were exposed to either 0.5 Gy or 5 Gy total body irradiation and analyzed seven days later. Representative dot plots of the gating strategies. A The population of CD11b + /GR1 + cells. B gMDSCs (CD11b + , Ly6G + , and Ly6C low ) and mMDSCs (CD11b + , Ly6G - , and Ly6C high ) from the spleens and intestines of irradiated mice were identified and analyzed by flow cytometry. C Representative dot plots of the gating strategy used to identify IL10 + cells. IL10 + cells were gated and the proportions of CD11b + /GR1 + cells. These cells were gated on gMDSCs (CD11b + , Ly6G + , and Ly6C low ) and mMDSCs (CD11b + , Ly6G − , and Ly6C high ) cells were evaluated from mouse spleens and intestines. D Representative immunofluorescence images of cells from spleen stained for GR1 (green), IL10 (red), and GR1/IL10 (yellow). DAPI staining (blue) was used to determine the number of nuclei. Scale bar = 100 μm. The data are represented as mean ± SD ( n = 8). ** P < 0.01, *** P < 0.001 vs. control.

Journal: Cell Death & Disease

Article Title: Expansion of monocytic myeloid-derived suppressor cells ameliorated intestinal inflammatory response by radiation through SOCS3 expression

doi: 10.1038/s41419-021-04103-x

Figure Lengend Snippet: Mice were exposed to either 0.5 Gy or 5 Gy total body irradiation and analyzed seven days later. Representative dot plots of the gating strategies. A The population of CD11b + /GR1 + cells. B gMDSCs (CD11b + , Ly6G + , and Ly6C low ) and mMDSCs (CD11b + , Ly6G - , and Ly6C high ) from the spleens and intestines of irradiated mice were identified and analyzed by flow cytometry. C Representative dot plots of the gating strategy used to identify IL10 + cells. IL10 + cells were gated and the proportions of CD11b + /GR1 + cells. These cells were gated on gMDSCs (CD11b + , Ly6G + , and Ly6C low ) and mMDSCs (CD11b + , Ly6G − , and Ly6C high ) cells were evaluated from mouse spleens and intestines. D Representative immunofluorescence images of cells from spleen stained for GR1 (green), IL10 (red), and GR1/IL10 (yellow). DAPI staining (blue) was used to determine the number of nuclei. Scale bar = 100 μm. The data are represented as mean ± SD ( n = 8). ** P < 0.01, *** P < 0.001 vs. control.

Article Snippet: The tissue sections were incubated with primary antibodies against CD11b (CST, 17800, 1:100 dilution, IL6 (CST, 12912, 1:100 dilution), IL10 (Abcam, ab192271, 1:100 dilution), and SOCS3 (Abcam, ab16030, 1:100 dilution), phospho-STAT3 (CST, 9145, 1:100 dilution), and GR1 (R&D Systems, MN, USA, MAB1037-100, 1:100 dilution) overnight at 4 °C.

Techniques: Irradiation, Flow Cytometry, Immunofluorescence, Staining, Control